ESTABLISHMENT OF A WELL-CHARACTERIZED SARS-COV-2 LENTIVIRAL PSEUDOVIRUS NEUTRALIZATION ASSAY USING 293T CELLS WITH STABLE EXPRESSION OF ACE2 AND TMPRSS2.

Establishment of a well-characterized SARS-CoV-2 lentiviral pseudovirus neutralization assay using 293T cells with stable expression of ACE2 and TMPRSS2.

Pseudoviruses are useful surrogates for highly pathogenic viruses because of their safety, genetic stability, and scalability for screening assays.Many different pseudovirus platforms exist, each with Wrinkle Serum different advantages and limitations.Here we report our efforts to optimize and characterize an HIV-based lentiviral pseudovirus assay

read more

Whole genome sequencing informs SNP-based breeding strategies to safeguard genetic diversity in captive African lions

IntroductionAfrican lions (Panthera leo) face severe population declines, making captive breeding programs essential for conservation.However, genetic data scarcity in such programs elevates inbreeding risks and threatens genetic diversity.MethodsUsing next-generation sequencing (NGS), we analyzed genome-wide genetic markers from 10 captive African

read more


Surface Tension Flows inside Surfactant-Added Poly(dimethylsiloxane) Microstructures with Velocity-Dependent Contact Angles

Filling of liquid samples Giftware is realized in a microfluidic device with applications including analytical systems, biomedical devices, and systems for fundamental research.The filling of a disk-shaped polydimethylsiloxane (PDMS) microchamber by liquid is analyzed with reference to microstructures with inlets and outlets.The microstructures are

read more